When you created the accdb file, was it in 2016?
Possibly there is a slight file format difference between them.
I havent used 2016, so cannot advise, however, previoius experience was that there might be a backwards compatible flag when creating, probably extends to 2010/2007.
2010 can on read only the newer format... That would be my guess.

You could try creating a front end/back end in 2010 and try opening it in 2016 to see if it still works or has a similar problem, or (hopefully not) fixes for 2016 and breaks for 2010...

post up what happens