region for TransparentColor(mask color)
Code:
Private Function MakeRegion(picSkin As PictureBox) As Long
    Dim x As Long, y As Long, StartLineX As Long
    Dim FullRegion As Long, LineRegion As Long
    Dim TransparentColor As Long
    Dim InFirstRegion As Boolean
    Dim InLine As Boolean
    Dim hdc As Long
    Dim PicWidth As Long
    Dim PicHeight As Long
    hdc = picSkin.hdc
    PicWidth = picSkin.ScaleWidth
    PicHeight = picSkin.ScaleHeight
    InFirstRegion = True: InLine = False
    x = y = StartLineX = 0
    TransparentColor = 14215660
    For y = 0 To PicHeight - 1
        For x = 0 To PicWidth - 1
            If GetPixel(hdc, x, y) = TransparentColor Or x = PicWidth Then
                If InLine Then
                    InLine = False
                    LineRegion = CreateRectRgn(StartLineX, y, x, y + 1)
                    If InFirstRegion Then
                        FullRegion = LineRegion
                        InFirstRegion = False
                    Else
                        CombineRgn FullRegion, FullRegion, LineRegion, RGN_OR
                        DeleteObject LineRegion
                    End If
                End If
            Else
                If Not InLine Then
                    InLine = True
                    StartLineX = x
                End If
            End If
        Next
    Next
    MakeRegion = FullRegion
End Function

Private Sub setrgn(picmainskin As PictureBox)
Dim WindowRegion As Long
    WindowRegion = MakeRegion(picmainskin)
    SetWindowRgn picmainskin.hwnd, WindowRegion, True
End Sub
this code make a mask color in picture box.
I want to delete a region