Changing root for project on the server

[venerable bede]

I have been developing a site in :

C:/inetub/wwroot/ and called it MySite. e.g "C:/inetub/wwroot/MySite"

It works great till I move it to the live server.
On the server I have put it in

C:/LiveSites/ and called it Live01 e.g "C:/LiveSites/Live01"
I then set up a virtual directory in IIS.

The problem is that when I open it in explorer it keeps looking for :
"C:/inetub/wwroot/MySite"

Can someone explain this to me ?