Help & Ideas Needed For Project Please

I work for a retail buy and sell shop and the shop seems to have acquired a large collection of video games over the years. About 3500 games total for just about every video game system out there. The boss wants to build a website where they can be for sale. I've just finished typing the title, price, genre, rating, description etc for each one of these games. I also have assigned a barcode number to these games and I have a barcode generator with a scanner that hooks inline with my keyboard. I can scan the bar code while excel is open, it translates the barcode into numbers, (000156) and prints it automatically wherever my cursor is on screen. For fast refrencing I usually just open the "find" box in excel and search for a game that way. I have it all saved in an excel database. There are a few things this website needs to be able to
1.) As new games are purchased they can be searched for locally. Once found, the quantity will be updated if it is in the database. Otherwise a new game will be entered. A barcode will be printed and the game is entered. At this point the database would be saved on our local server here and the website is then updated with that title.
2.) I am thinking that the website needs to be able to query the database if a customer searches for a game. A list of possible matches appears and so on and so forth. A good example of what we are trying to do is at: www.gamestop.com
what is the best way to write code that dynamically creates a page for a video game based on the database descriptions. It seems to me that the website would use a template where predefined bits of information are pulled from the database. "Description" of the game for example will be sucked form the database and put into a certain area of the dynamically created page. Does the server suck from the database here at the store every time a query is made? or does it keep it's own copy of the database on the server?

I'm not a highly experienced programmer but I've never not been able to figure something out. A little help in the right direction would be great. I am most comfortable with VB or VB.net.