MS Access database connection problem

I am trying to connect to an MS Access database on our web server using ASP.NET. I keep getting the message:

"The Microsoft Jet database engine cannot open the file
'Webservername\Filename.mdb'. It is already opened exclusively by another user, or you need permission to view its data."

I have set up the ASPNET account on the folder in the virtual directory, as well as the mdb file, and I have closed my VB.Net project. (It seems to create an .ldb file if you leave it open after creating an oledb connector)

Has anyone run into this issue before?

might it be that you do not properly dispose of the connections or that you open the db connection and experience a bug (and therefore it remains open)?

is this coming also after a proper reboot?